Fig. 2. scRNA-seq of HUCMSCs reveals four MSC clusters and a transcriptome enhanced for regeneration, anti-inflammation, cell cycle and metabolism.
a, Uniform manifold approximation and projection (UMAP) graph of the first two UMAP dimensions shows four clusters of MSCs. Based on the pathway/GO annotation of their marker genes, the functional labels were added to the graph. MSC-like cells were isolated from the human umbilical cord (Wharton’s jelly) after delivery of a full-term infant (38-week gestation). After MSC culture, MSCs were harvested in passage 3 to undergo scRNA-seq, as described in the Methods. b, LC–MS chromatogram showing the mass spectrometric traces of PGE2, PGF2α and the internal standard d4-PGE2. c–f, EDTA plasma concentrations of NEDD9, ICAM-1, SAA and IFN-γ (mean of concentrations near-simultaneously measured in the SVC, IVC and RA). AU, arbitrary units.