Figure 2.

Protein expression of clock components of the positive and negative arm of the circadian clock in the rat lens. 20µg/lane of positive control tissue (kidney, liver or brain (n= 1 rat)) and 20µg/lane lens tissue (n=8 rats) was electrophoresed on an SDS-PAGE gel and protein levels analysed by Western blotting. Expression of BMAL1, CLOCK, PER1, PER2, PER3, CRY1 & CRY 2. Arrowheads indicate the predicted size of the target protein. Target proteins were all identified in positive control tissue but not all could be detected in the lens.