Figure 4.

Relative expression of NRF2 and NRF2 related proteins in the rat lens over a 24-hour period. Lens tissue (n=8 rats) was harvested at 4-hour intervals over a 24-hour period. 20µg lens protein/lane was electrophoresed on an SDS-PAGE gel and protein levels were analysed by Western blotting and expressed relative to the β-actin internal control. Relative expression of (A) NRF2, (B) GCLC, (C) GS and (D) GR in lenses at different time points. Data is presented as mean relative expression ± standard error of the mean (SEM) from 5-7 western blots. * Indicates significant differences from the 6am time point; *p<0.05, ***p<0.001.