Figure 5.

Expression of clock component proteins and NRF2 in the rat lens at different times of the day. Subcellular localisation of BMAL1, CLOCK and NRF2 at different times of the day. Lenses were dissected at 10am, 2pm, 6pm or 10pm, fixed, cryoprotected and cryosectioned in an axial orientation. Images were taken at either the anterior pole (A–L) or the lens equator (A’-L’). (A–L) Left hand panels-sections labelled with the membrane marker WGA (red), DAPI (blue) and BMAL1, CLOCK or NRF2 antibodies (green). A’-L’: Right hand panels -sections showing only BMAL1, CLOCK or NRF2 labelling. n = 4 lenses.