Fig. 4. TOPORS-KO MDS/AML cells show increased apoptosis and mitotic defects upon DAC treatment.

a Frequency of apoptotic cell death in WT and TOPORS-KO MDS-L cells after exposure to 12.5 nM of DAC. Representative flow cytometric profiles of cells at 72 h of DAC exposure (left panel). Percentage of Annexin V-positive cells (n = 3 for each group, technical replicates, the experiments were repeated twice independently) (right panel). b Cell cycle status of WT and TOPORS-KO MDS-L cells after exposure to 12.5 nM of DAC evaluated by EdU incorporation and DAPI staining. Representative flow cytometric profiles of cells at 72 h of DAC exposure (left panel). Proportion of each cell cycle (middle panel) and hyperploidy cells at the indicated time points (right panel) (n = 3 for each group, technical replicates, the experiments were repeated twice independently). Data are presented as mean ± SD. Source data are provided as a Source Data file.