Fig. 3. Comparison of FTEC proliferation between the initial culture and after 3 passages as a function of viscosity at 72-hour time point for each passage.

a Representative Ki67 positive staining images of cells at the 72-h time point for passages 1 and 3, and the b percentage Ki67 positive cells. Scale bar, 30 µm. Experiments were carried out on n = 3 from three biological replicates and ≥ 200 cells for each condition were analysed. Data are represented as mean ± s.d. and analysed using a two-tailed t-test, *P ≤ 0.05 (P = 0.0131). Source data are provided as a Source Data File.