Fig. 2. Ad26.M.Env vaccine induces low level cellular immune anti-Env responses in pregnant macaques.

IFNγ ELISPOT responses in PBMCs of dams (n = 9) immunized with 10¹¹ vp Ad26.M.Env (a, c) or non-immunized control animals (n = 5) (b, d). a, b Env-specific responses and (c, d) PrM specific responses are shown. The geometric mean response per group is indicated with a horizontal line. Responses above 50 SFU per 10⁶ PBMCs indicated by the dotted line are considered positive. Individual animals of group 1 (Ad26.M.Env immunized) are color coded to represent number of weeks between immunization and challenge; green (10wk); purple (12 weeks); blue (14 weeks); gray (18 weeks); red (22 weeks); brown (30 weeks). a Friedman one-way ANOVA, followed by Dunn’s test for multiple comparisons; *p = /< 0.05; **p = /< 0.01; ***p = < 0.001. Samples below LOD were set to 50.