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. 2024 Aug 20;6(3):475–487. doi: 10.1007/s42995-024-00239-9

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© The Author(s) 2024

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Fig. 1 — Validation of the recombinant P. pastoris GS115-NTaer strain and safety of the antigen. A PCR analysis of P. pastoris genome and WB analysis of proteins secreted into medium. B Hemolysis test on blood plate. C Hemolysis rate of different concentrations of aerolysin Hmaer and its mutant NTaer. In two-group comparisons, significant differences were marked with ** (P < 0.01) or **** (P < 0.0001)