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Effect of vaccine on gut epidermal physical barrier and immunological response. A–D Relative expression of genes associated with tight junctions. E–G Content of lysozyme (LYZ), complement C3 and C4 in gut. H–N Profile of immune-related gene expression post vaccination. Data represent the means (± SD) of six replicates of each group, groups with different letters indicate significant differences (P < 0.05), P between 0.05 and 0.1 was considered trend
