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. 2024 Aug 20;6(3):475–487. doi: 10.1007/s42995-024-00239-9

Table 2.

List of qRT-PCR primers used in this study

Gene Forward primer (5′-3′) Reverse primer (5′-3′) Size (bp) Accession no.
β-actin ATGGATGAGGAAATCGCTG ATGCCAACCATCACTCCCTG 140 AF057040.1
Occludin-b TGGAGATGAGCTTGACACAGATG CCTTCCTCTAGCCTGTCGAG 177 BC164682.1
Claudin7 GGAGCCCTCTGTAGCATTGTT ATTAGGAGATGACTGACCCTTTGA 198 BC066408.1
Claudin11a ACCAGAAAGTGCCAAGAACA AAAGCCAAAGGACATCAGACC 136 NM_001002624
TJP1a CAAAGACCAACAGCACTGCC GTGGTTTAGCGGTGATGGGA 177 XM_021477863
C4 AGGGAACAGGACGGGGAAC GTCGGTTTTGTACTCCAGCTCT 125 XM_005157372.4
C3a ATGAGCTCCTGCAGAGGTGT AGTGGTTGTTGGAGGTCTGG 179 XM_005174032
LYZ GATTTGAGGGATTCTCCATTGG CCGTAGTCCTTCCCCGTATCA 103 NM_139180
MHCII ACAGGGACTGATATTTGCA CAGCCTGCCTCACTGACTTG 167 NM_131476
CD4 GTGTTTGGACATGCCAGTTG AAGCACAGGGAATGCTGACT 144 HE983359.2
CD8α AGGTTGTGGACTTTTCCTCGT GGAGCTAGAAGTGGCTGGTG 149 BC162235.1
T-bet GCAGCTCCAACAACGTAGCA ATCCTCCTTCACCTCCACGATG 89 NM_001170599.2
GATA3 GGTGAGATGTAGGGAGAGGAAACC TGCCCAAGACCTATAACACATCCA 120 XM_005164809
TCRα CTTAAAACGTCGGCTGTCCG TGAACAAACGCCTGTCTCCT 93 AY476727.1
MPEG1 ATGTGGATTCCCCAAACTTCAACT TGGTAAATGCCACCAAAGCTAAGA 94 CP137045.2