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. 2024 Aug 13;24(16):5241. doi: 10.3390/s24165241

Table 1.

Salmon aquaculture pathogens selected for qPCR assay development.

Pathogen DSM
No.
Target
Gene
Primer/Probe 5′-3′
(F: Forward Primer; R: Reverse Primer; P: Probe Sequence)
Aeromonas salmonicida
(A. sal)
19634 vapA F: TTTCTGGCGTAGGCCGTTTA
R: CTTCCGAACGTCATTAACTTCACC
P:/6-FAM/CTGCTGGTT/Zen/AACCCGAATGATCATGGTAAT/IABkFQ/
Vagococcus salmoninarum
(V. sal)
6633 pheS F: AGAGCGGGAAATTCGTCTTC
R: GGGAAGGCTGTAACGTTTGTA
P:/SUN/ACTGAACCT/Zen/TCCGTCGAAGTTGATGT/IABkFQ
Yersinia Ruckeri
(Y. ruc)
18506 glnA F: TCCAGCACCAAATACGAAGGT
R: GATCTGCGTTCTGCCATGT
P:/HEX/CCCAGTTGA/Zen/TTCCGCGCAAGATCT/IABkFQ/