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. 2024 Jul 23;12(8):832. doi: 10.3390/vaccines12080832

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CSFV RNA detection by the RT-qPCR test in foetuses from vaccinated and non-vaccinated sows after CSFV challenge. Samples from foetuses were evaluated by the specific CSFV Margarita strain and the generic CSFV RT-qPCR assays. Results are shown in a colourimetric scale representing the range of values for the detection of both CSFVs: low for Ct values of 29 or above, moderate for Ct between 28 and 23, and high for values under 22. The results obtained are represented in red and blue for the specific CSFV Margarita and generic CSFV tests, respectively. The symbol “−” represents a negative result. In litter number 5, only one serum sample could be evaluated since the other foetuses in the litter were mummified after CSFV infection.