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Two electrophoresis conditions tested with five representative Shigella flexneri strains run in a single gel with the restriction enzymes XbaI (lines 3, 6, 8, 11, 13, and 14) and NotI (lines 2, 4, 7, 9, and 12). SB, PulseNet universal size standard Salmonella enterica serotype Braenderup (H9812). (Left panel) Program 3, 10–25 s. (Right panel) Program 7, 5–35 s.
