Table 2.
(A) Electrophoresis Conditions Evaluated in Phase I with Shigella flexneri Strains; (B) Electrophoresis Conditions Tested with a Selection of Seven Strains of Shigella flexneri During Phase II/Final Standardization Trials
| (A) | ||
|---|---|---|
| PulseNet Protocol | Electrophoresis conditions a | Reference |
|
| ||
| Escherichia coli O157:H7, Shigella sonnei | 2.2–54.2 s for 18 h | Ribot et al., 2006. |
| Yersinia pestis modified | 1.8—25 s for 18 h | www.cdc.gov/pulsenet/protocols.htm |
| (B) | |
|---|---|
| Program | Electrophoresis conditions a |
|
| |
| Program 1 (P1) | Block 1: 2–10 s for 12h Block 2: 20—25 s for 5 h |
| Program 2 (P2) | Block 1: 2—5 s for 12h Block 2: 20—25s for 5h |
| Program 3 (P3) | 10—25 s for 17.5 h |
| Program 4 (P4) | 2—30 s for 17.5 h |
| Program 5 (P5) | 10—35 s for 17.5 h |
| Program 6 (P6) | 5—25 s for 17.5 h |
| Program 7 (P7) | 5—35 s for 17.5 h |
a
Run time indicated is approximate and was adjusted in each lab to achieve adequate migration of the standard strain DNA fragments.