Table 2.
(A) | ||
---|---|---|
PulseNet Protocol | Electrophoresis conditions a | Reference |
| ||
Escherichia coli O157:H7, Shigella sonnei | 2.2–54.2 s for 18 h | Ribot et al., 2006. |
Yersinia pestis modified | 1.8—25 s for 18 h | www.cdc.gov/pulsenet/protocols.htm |
(B) | |
---|---|
Program | Electrophoresis conditions a |
| |
Program 1 (P1) | Block 1: 2–10 s for 12h Block 2: 20—25 s for 5 h |
Program 2 (P2) | Block 1: 2—5 s for 12h Block 2: 20—25s for 5h |
Program 3 (P3) | 10—25 s for 17.5 h |
Program 4 (P4) | 2—30 s for 17.5 h |
Program 5 (P5) | 10—35 s for 17.5 h |
Program 6 (P6) | 5—25 s for 17.5 h |
Program 7 (P7) | 5—35 s for 17.5 h |
Run time indicated is approximate and was adjusted in each lab to achieve adequate migration of the standard strain DNA fragments.