Figure 1.

Construction and confirmation of transgenic Arabidopsis thaliana lines. The pGMT-GSL5 and pGMT-GFP were two plasmids containing the amplified GSL5 promoter region and gfp coding sequence, respectively, in the pGMT-vector. The pGMT-GSL5::GFP was obtained by the insertion of the GSL5 promoter region to the pGMT-GFP. The DNA fragment from EcoRI to SacI in the pGMT-GSL5::GFP was used to replace that from HindIII to SacI in pBI121 to obtain pBI121-GSL5::GFP. Electrophoresis was carried out to confirm the correct size of the constructed fragments by PCR amplification with specific primers. Marked above and below the sequence are the corresponding primers and restriction enzyme sites, respectively.