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. 2024 Aug 7;13(16):2185. doi: 10.3390/plants13162185

Figure 3.

Figure 3

Screening of the transgenic Arabidopsis thaliana AtGSL5-GFP homozygous line. (A) shows the seedings of the possible transgenic A. thaliana AtGSL5-GFP lines (#1, #2, and #4) obtained from the seedlings grown on 1/2 MS medium with kanamycin. (B) indicates the specific amplicon amplified from the genomic DNA of AtGSL5-GFP lines. (C) shows the seedlings of the possible homozygous AtGSL5-GFP line 1–13 grown on 1/2 MS medium with kanamycin. (D) indicates the homozygous AtGSL5-GFP line 1–13 confirmed by the PCR amplification. The PCR amplification was performed with specific primer GFPPF/PBI-IndR to confirm the DNA fragment. M indicates the DNA ladders (Genemark, Taichung, Taiwan).