Table 2. Composition of Gels Used for Native PAGE, SDS-PAGE and Urea-PAGE^a.

	Native PAGE	SDS-PAGE	Urea-PAGE
Running (resolving) gel
Deionized water (Milli-Q)	3.7 mL	3.7 mL	Make up to 10 mL
30% acrylamide	3.3 mL	3.3 mL	3.3 mL
10% bis-acrylamide	330 μL	330 μL	330 μL
Tris-HCl (1.5M, pH 8.8)	2.5 mL	2.5 mL	2.5 mL
10% SDS		100 μL
Urea (6 M final conc.)			3.6 g
10% ammonium persulfate	100 μL	100 μL	100 μL
TEMED	6 μL	6 μL	6 μL
Stacking gel
Deionized water (Milli-Q)	2.7 mL	2.7 mL	Make up to 4 mL
30% acrylamide	670 μL	670 μL	670 μL
10% bis-acrylamide	67 μL	67 μL	67 μL
Tris-HCl (1.5 M, pH 6.8)	500 μL	500 μL	500 μL
10% SDS		40 μL
Urea (6 M final conc.)			1.4 g
10% ammonium persulfate	40 μL	40 μL	40 μL
TEMED	4 μL	4 μL	4 μL
Running buffer (10×)
Tris base	30.3 g	30.3 g	30.3 g
Glycine	144 g	144 g	144 g
SDS		10 g
Urea (6 M final conc.)			360 g
Deionized water (Milli-Q)	Make up to 1 L	Make up to 1 L	Make up to 1 L

^aSDS refers to sodium dodecyl sulfate. TEMED refers to tetramethylethylenediamine.