Figure 5.

EPZ6438 treatment partially restores reduced slow wave amplitudes and inhibitory neuromuscular transmission in klotho mice. (A) Representative traces show diminished slow wave amplitudes in the corpus and antrum of klotho mice relative to WT control animals. EPZ6438 treatment led to partial restoration of these amplitudes in klotho mice. (B) Illustration of separation of fast and slow inhibitory junction potentials (IJPs). (C) Representative recordings of electrical responses to electrical field stimulation (square pulses delivered by parallel platinum electrodes; 0.3 ms; 5–20 Hz for 1 second; supramaximal voltage). EPZ6438 treatment led to partial restoration of fast and slow IJPs in klotho mice. Quantitative analysis of fast and slow IJPs and excitatory junction potentials is shown in Table 1. Statistical significance was determined using Kruskal-Wallis 1-way analysis of variance (on ranks). ∗P < .05, ∗∗P < .01.