Figure 7.

Genomic deletion of Ezh2 in Kit-positive ICC lineage preserved ICC of Kit^creERT2/+;Ezh2^fl/flmice with age. (A) Tamoxifen (Tam; 0.075 mg/g intraperitoneally for consecutive 3 days at 18 months of age)-induced recombination restored reduced KIT protein of Kit^creERT2/+;Ezh2^fl/fl mice compared with vehicle (Veh)-treated control mice. Tam-treated Kit^creERT2/+;Ezh2^+/+ mice were served as alternative control. n = 5/group. (B) Reduced gastric ICC networks in Kit^creERT2/+;Ezh2^fl/fl mice were restored by Tam treatment. Representative confocal stacks showing KIT⁺ (green) and ANO1⁺ (magenta) myenteric ICC (ICC-MY) and intramuscular ICC (ICC-IM) in corresponding regions of the gastric corpus (greater curvature, full thickness) of a Kit^creERT2/+;Ezh2^fl/fl and Kit^creERT2/+;Ezh2^+/+ mice. n = 5–6/group. Scale bar: 50 μm. Statistical significance was determined using Kruskal-Wallis 1-way analysis of variance (on ranks). ∗P < .05, ∗∗P < .01. ns, not significant.