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. 2024 Jul 3;18(4):101376. doi: 10.1016/j.jcmgh.2024.101376

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Quantitative image analysis workflow diagrams. (A) GA3 recipe for setting background removal, AI-based segmentation, volume quantification, and export. (B) GA3 recipe for obtaining the volumes of KIT (enhanced green fluorescent protein; eGFP) or ANO1 (cyanine 5; Cy5) within the flattened segmented areas of KIT (eGFP) or ANO1-positive intramuscular ICC (ICC-IM), which are used for AI training. (C) GA3 recipe for obtaining the volumes of KIT (eGFP) or ANO1 (Cy5) within flattened segmented areas of KIT (eGFP) or ANO1-positive myenteric ICC (ICC-MY) that are used for AI training.