Table 1.
Parameters of Postjunctional Electrical Responses to EFS in WT, Veh-Treated klotho Mice, and EPZ-treated klotho Mice
| EFS frequency | Parameter | WT Veh |
klotho Veh |
klotho EPZ | P (WT Veh vs klotho Veh) | P (WT Veh vs klotho EPZ) | P (klotho veh vs klotho EPZ) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| 5 Hz | Fast IJP | 2.214 ± 0.6908 | [0.5573; 1.510; 3.939] (9) | 0.2890 ± 0.1692 | [0.000; 0.000; 0.2654] (16) | 0.3230 ± 0.1328 | [0.000; 0.09462; 0.7452] (10) | .001 | .0033 | .9971 |
| Slow IJP | 0.9944 ± 0.3020 | [0.3431; 0.8320; 1.937] (8) | 0.3828 ± 0.1998 | [0.000; 0.000; 0.5364] (16) | 0.3856 ± 0.2173 | [0.000; 0.000; 0.7044] (10) | .1839 | .2436 | > .9999 | |
| Fast + slow IJP | 3.919 ± 1.305 | [1.113; 2.349; 5.971] (9) | 0.6323 ± 0.3415 | [0.000; 0.000; 0.6194] (17) | 0.7087 ± 0.3354 | [0.000; 0.2506; 1.155] (10) | .0031 | .0099 | .9959 | |
| 10 Hz | Fast IJP | 5.227 ± 1.564 | [1.749; 2.836; 9.272] (9) | 1.375 ± 0.4402 | [0.04487; 0.7626; 2.098] (16) | 2.193 ± 0.5199 | [1.199; 1.702; 2.646] (10) | .0061 | .0596 | .7472 |
| Slow IJP | 3.763 ± 1.083 | [1.050; 2.606; 6.093] (9) | 1.456 ± 0.4640 | [0.07564; 0.5321; 2.768] (15) | 3.239 ± 0.7012 | [1.602; 2.789; 4.039] (10) | .0693 | .8804 | .1725 | |
| Fast + slow IJP | 8.990 ± 2.604 | [3.351; 4.577; 14.54] (9) | 2.580 ± 0.7995 | [0.2551; 1.111; 4.958] (15) | 4.340 ± 0.4327 | [3.356; 3.807; 5.365] (9) | .0068 | .0982 | .6403 | |
| 20 Hz | Fast IJP | 5.393 ± 1.282 | [3.355; 4.720; 6.090] (8) | 1.801 ± 0.6435 | [0.000; 0.5436; 2.647] (15) | 3.860 ± 1.026 | [1.047; 3.616; 6.573] (10) | .0284 | .5399 | .233 |
| Slow IJP | 7.926 ± 2.614 | [2.818; 3.843; 12.47] (9) | 2.857 ± 4.228 | [0.04420; 0.5175; 5.418] (16) | 5.932 ± 1.611 | [1.205; 5.260; 9.252] (10) | .0895 | .7184 | .3689 | |
| Fast + slow IJP | 11.24 ± 2.259 | [7.369; 7.788; 18.38] (8) | 4.004 ± 1.369 | [0.000; 0.8982; 9.647] (15) | 9.792 ± 1.494 | [5.857; 10.67; 13.32] (10) | .0128 | .8409 | .0357 |
NOTE. Responses to square wave electrical pulses (0.3 ms; 5–20 Hz for 1 second; supramaximal voltage) are expressed as areas under the curve (mV) (ie, the areas segmented by RMP before electrical simulation and the subsequent junction potentials). Data are mean ± and [median + interquartile range] from 3 WT, 6 klotho Veh, and 5 klotho EPZ. The number of responses that were analyzed are located in parentheses.
EFS, electrical field stimulation; EPZ, EPZ6438; IJP, inhibitory junction potential; Veh, vehicle; WT, wild-type.