Table 3.
Antibodies Used for Flow Cytometry Analysis of Cells Freshly Dissociated from Murine Gastric Muscles
| Target | Supplier | Host/Source | Clone/ID | Isotype | Label | Final conc. or μg/106 cells |
|---|---|---|---|---|---|---|
| CD16/32 | eBioscience | Rat mAb | 93 | IgG2a, λ | 1 μg | |
| CD11b | eBioscience | Rat mAb | M1/70 | IgG2b, κ | PE-Cy7 | 0.0312 μg |
| CD45 | eBioscience | Rat mAb | 30-F11 | IgG2b, κ | PE-Cy7 | 0.0312 μg |
| F4/80 | eBioscience | Rat mAb | BM8 | IgG2a, κ | PE-Cy7 | 0.0625 μg |
| CD44 | BioLegend | Rat mAb | IM7 | IgG2b, κ | APC-Cy7 | 0.0625 μg |
| KIT | eBioscience | Rat mAb | ACK2 | IgG2b, κ | APC | 5 μg/mL |
| KIT | eBioscience | Rat mAb | 2B8 | IgG2b, κ | APC | 0.25 μg |
| CD34 | eBioscience | Rat mAb | RAM34 | IgG2a, κ | eFluor 450 or FITC | 0.2 μg |
FITC, fluorescein isothiocyanate; mAb, monoclonal antibody.