Table 5.
Antibodies Used in WB Studies
| Target | Supplier | Host | Clone/ID | Isotype/lot # | Label | Final conc. |
|---|---|---|---|---|---|---|
| ANO1 | Abcam (Cambridge, MA) | Rabbit pAb | Ab53212 | IgG/GR3295356-1 | 1:1000 | |
| EZH2 | CST (Beverly, MA) | Mouse mAb | 3147 | IgG/4 | ||
| GAPDH | Novus | Goat pc | IMG-3073 | C3G2 | 0.05 μg/mL | |
| GAPDH | Sigma | Rabbit pAb | G9545 | IgG1/099M4801V | 1:40000 | |
| KIT | R&D Systems | Goat pAb | AF1356 | IgG/ IEO0217101 | 0.2 μg/mL for mice | |
| KIT | DAKO (Carpinteria, CA) | Rabbit pAb | A4502 | 100428020A | 1:4000 for human beings | |
| H3K27me3 | CST | Rabbit mAb | 9733 | IgG/8 | 1:2000 | |
| H3 | Thermo Fisher | Mouse mc | 865R2 | IgM/I293050 | 0.5 μg/mL | |
| SCF | RDS | Goat pAb | AF-455-NA | IgG/AIL0323011 | 0.1 μg/mL | |
| T-TRP53 | CST | Mouse mAb | 2524 | IgG1/17 | 1:2000 | |
| P-TRP53 (Ser15) | CST | Rabbit pAb | 9284 | 12 | 1:1000 | |
| Secondary Ab: Anti-rabbit IgG (H + L) |
LI-COR | Donkey pAb | #926-32223 | C90821-03 | IRDye 680 | 1:10000 |
| Secondary Ab: Anti-mouse IgG (H + L) |
LI-COR | Donkey pAb | #926-32222 | C71204-03 | IRDye 680 | 1:10000 |
| Secondary Ab: Anti-rabbit IgG (H + L) |
LI-COR | Donkey pAb | #926-32213 | C70918-03 | IRDye 800CW | 1:10000 |
| Secondary Ab: Anti-goat IgG (H + L) |
LI-COR | Donkey pAb | #926-32214 | C80207-07 | IRDye 800CW | 1:10000 |
Ab, antibody; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; H + L, highly cross-absorbed; mAb, monoclonal antibody; pAb, polyclonal antibody; SCF, stem cell factor; WB, Western immunoblotting.