Table 6.
Target | Supplier | Host | Clone/ID | Isotype/lot # | Label | Final conc. |
---|---|---|---|---|---|---|
ANO1 | Abcam | Rabbit pAb | ab53212 | IgG/GP3295656-1 | 1:1500 | |
KIT | R&D Systems | Goat pAb | AF1356 | IgG/IEO0217101 | 0.2 μg/mL | |
Secondary Ab: Anti-rabbit IgG | Life Technologies (Grant island, NY) | Donkey pAb | A31573 | 2577247 | AF647 | 5 μg/mL |
Secondary Ab: Anti-goat IgG | Life Technologies | Donkey pAb | A11055 | 2411589 | AF488 | 5 μg/mL |
Ab, antibody; pAb, polyclonal antibody.