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. Author manuscript; available in PMC: 2024 Aug 29.
Published in final edited form as: Biochem Pharmacol. 2021 Sep 21;193:114781. doi: 10.1016/j.bcp.2021.114781

Fig. 4. The alternative ZAC/m5-HT3A-II and hα1-Gly/ZAC-II chimeras and the ECD/TMD-ICD chimeras with Cys-loop and/or C-terminus modifications.

Fig. 4.

A. Topologies of the chimeric ZAC/m5-HT3A-II and hα1-Gly/ZAC-II subunits and illustration of the pentameric complexes assembled from them. The amino acid sequences of the ECD-into-TMD sequences in the three WT subunit proteins are given, and the alternative fusion points in ZAC/m5-HT3A-II and hα1-Gly/ZAC-II (“fusion-II”) compared to those in the original ZAC/m5-HT3A and hα1-Gly/ZAC chimeras (“fusion”) are indicated. B. Schematic outline of the modifications made to the PFX-motif in the Cys-loop and in the C-terminal in the ECD-parts and the TMD/ICD-parts of the ZAC/m5-HT3A, m5-HT3A/ZAC, ZAC/hα1-Gly and hα1-Gly/ZAC chimeras.