Fig. 4.

Control, dECM, EVs and dECM + EVs group induce chondrogenesis differentiation. A-D) Fluorescence microscope images of BMSCs in Control, dECM, EVs and dECM + EVs group detected via immunofluorescence labeling 21 d after cell seeding. Upper panels show “merged” (protein expression + nucleus + F-actin) staining and lower panels show protein expression. E-K) Quantitative evaluation of protein expression via ImageJ software. The results are expressed as protein expression area/total area × 100 (%). Data represent the mean ± standard deviation (Scale Bar: 50 μm) (*P < 0.05 compared with other groups).