Table 2.
Percent germination, germination index, vigor index, amylase activity, H2O2 content, APX activity, and GR activity in 5-days-old mustard (Brassica juncea L. cv. Pusa Vijay) seedlings grown under normal or 100 mmol NaCl stress conditions after seeds were pretreated with 2.0 mmol Eth, 25 µmol ABA, 25 µmol Flu, or 0.1 µmol AVG. Values are presented as means ± SE (n = 4). Data means followed by the same letter are not significantly different at p ≤ 0.05 according to the LSD test. ABA: abscisic acid; APX: ascorbate peroxidase; AVG: aminoethoxyvinylglycine; Eth: ethephon; GR: glutathione reductase; Flu: fluoridone.
| Treatment | Germination Percentage (%) |
Germination Index (%) |
Vigor Index (%) |
Amylase Activity (U g−1 Protein min−1) |
H2O2 Content (nmol g−1 FW) |
APX Activity (U g−1 Protein min−1) |
GR Activity (U g−1 Protein min−1) |
|---|---|---|---|---|---|---|---|
| Control | 72 ± 1.9 b | 21.2 ± 0.67 b | 3.2 ± 0.10 b | 82.5 ± 2.4 b | 46.3 ± 1.2 f | 0.68 ± 0.02 g | 0.27 ± 0.01 f |
| NaCl | 46 ± 1.2 e | 10.5 ± 0.299 e | 0.9 ± 0.02 e | 44.7 ± 1.2 e | 198.4 ± 5.4 a | 1.07 ± 0.03 e | 0.33 ± 0.02 e |
| Eth | 82 ± 2.4 a | 24.8 ± 0.72 a | 3.6 ± 0.11 a | 105.6 ± 3.3 a | 31.5 ± 0.8 h | 2.25 ± 0.06 c | 0.46 ± 0.03 c |
| ABA | 21 ± 0.4 f | 6.3 ± 0.72 f | 0.7 ± 0.02 f | 39.4 ± 1.0 f | 38.2 ± 0.9 g | 2.06 ± 0.06 d | 0.36 ± 0.01 de |
| Eth + NaCl | 58 ± 1.5 d | 14.2 ± 0.68 d | 1.1 ± 0.04 d | 66.8 ± 2.1 d | 53.7 ± 1.4 e | 3.41 ± 0.08 a | 0.67 ± 0.03 a |
| ABA + NaCl | 13 ± 0.1 g | 1.7 ± 0.04 g | 0.5 ± 0.01 g | 38.9 ± 0.6 fg | 71.8 ± 1.9 c | 2.21 ± 0.06 cd | 0.53 ± 0.01 bn |
| ABA + NaCl +AVG |
7 ± 0.3 h | 1.3 ± 0.05 h | 0.3 ± 0.01 h | 23.2 ± 1.0 h | 81.2 ± 1.9 b | 0.84 ± 0.02 f | 0.29 ± 0.01 fg |
| Eth + NaCl + Flu |
60 ± 1.4 c | 15.2 ± 0.39 c | 1.5 ± 0.03 c | 76.5 ± 1.8 c | 62.3 ± 1.4 d | 2.82 ± 0.09 b | 0.56 ± 0.01 b |