Fig. 5.

Triptolide induces copper homeostasis imbalance by regulating the XIAP/COMMD1 pathway. A, B Relative mRNA levels of ATP7A, ATP7B and CTR1 in HeLa and SiHa cells treated with or without triptolide for 48 h. C Venn diagram of triptolide, cervical cancer and copper homeostasis targets. D Visualization of 27 common targets. E Molecular docking between XIAP and triptolide. F, G The protein content of HeLa cells was analyzed after treatment with 80 nM triptolide for 48 h. H, I The protein content of SiHa cells was analyzed after 40 nM triptolide treatment for 48 h. (*p < 0.05, **p < 0.01, ***p < 0.001 versus the CON group; ns, not significant.)