TABLE 3.

Evaluation survey content

IDa	Question text	Possible answersb
A	How old are you?
B	Do you know what a bacterium is?	y/n
C	Do you know what a bacterial capsule is?	y/n
D	Do bacteria have the tools to harm each other?	y/n/idk
E	Do bacteria reproduce at the same pace?	y/n/idk
F	What is sporulation?	A resistant state that some bacteria can achieve under unfavorable conditions.
		The release of toxins by bacteria.
		idk
G	What are flagella used for?	Sticking to surfaces.
		Motility in liquid environments.
		idk
H	What does it mean to be lithotrophic?	A bacterium can get energy from minerals.
		A bacterium can get energy from the sunlight.
		idk
I	Can bacteria be infected by viruses?	y/n/idk
J	Are all bacteria harmful to humans?	y/n/idk
K	How many bacteria are in a coffee spoon of yoghurt?	Millions
		Hundreds
		idk
L	How easy did you find the gameplay?	VE/E/A/D/VD
M	Did you find the card content easy to understand?	VE/E/A/D/VD
N	Did you like the setup of the game?	y/n/idk
O	Would you like to play this game again?	y/n/idk
P	What can we improve?

^a Question A categorizes the player’s age; B and C assess the initial level of knowledge in microbiology (none, both questions are answered negatively; basic, player knows what a bacterium is but not a bacterial capsule; or advanced, both answers are positive); questions D–I score knowledge acquisition; J and K are control questions; L–O evaluate the appreciation of the game; and P is an optional free-text entry answer for additional feedback.

^b y = yes, n = no, idk = I don’t know, VE = very easy, E = easy, A = adequate, D = difficult, VD = very difficult.