Table.
Primary Antibodies
| Primary Antibody | Host | Source | Dilution | Müller Glia | Astrocytes | RPE Cells | Amacrine Cells | Bipolar Cells | Plug |
|---|---|---|---|---|---|---|---|---|---|
| GFAP | Rabbit | Dako no. Z033429-2 | 1:2000 | + | + | − | − | − | + |
| S100B | Mouse | Sigma-Aldrich no. S2532 | 1:500 | − | + | − | − | − | + |
| GS | Rabbit | Sigma-Aldrich no. G2781 | 1:1000 | + | − | − | − | − | − |
| CK-AECAM | Mouse Rabbit | Agilent no. IR053 no. IR094 | 1:1 | − | − | + | − | − | − |
| PKCα | Mouse | Sigma-Aldrich no. P5704 | 1:500 | − | − | − | − | + | |
| TH | Rabbit | Chemicon no. AB152 | 1:500 | − | − | − | + | − |
Primary antibodies used to identify Müller glia, astrocytes, neuroepithelial cells (RPE), amacrine cells, and bipolar cells in the porcine retina. Positive and negative staining of the cells is shown with “+” and “−.“ Markers found in the retinal plug are seen in the column on the far right.