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. 2024 Aug 29;19(8):e0304839. doi: 10.1371/journal.pone.0304839

Fig 3. B. burgdorferi DUF490 interacts with the POTRA domains of BamA.

Fig 3

Lower molecular weight bands (26 kDa) in anti-GST lanes correspond with the size of the GST tag. The black arrows next to blots indicate the GST fusion band and white arrows indicate the 6xHis fusion band. The colored arrows below the blots indicate proteins expressed in each assay and the expected molecular weights are listed here. A. Whole cell lysate and co-purification from E. coli expressing BbDUF490 (DUF, 66 kDa) as well as BbPOTRA1-5 (P1-5, 45 kDa), subjected to immunoblotting with anti-GST antibody for BbDUF490 and anti-6xHis antibody for BbPOTRA1-5. B. Whole cell lysate and co-purification from E. coli expressing BbDUF490 Segment 1 (S1, 39 kDa) as well as BbPOTRA1-5, subjected to immunoblotting with anti-GST antibody for Segment 1 and anti-6xHis antibody for BbPOTRA1-5. C. Whole cell lysate and co-purification from E. coli expressing BbDUF490 Segment 2 (S2, 39 kDa) as well as BbPOTRA1-5, subjected to immunoblotting with anti-GST antibody for Segment 2 and anti-6xHis antibody for BbPOTRA1-5. D. Whole cell lysate and co-purification from E. coli expressing BbDUF490 Segment 3 (S3, 39 kDa) as well as BbPOTRA1-5, subjected to immunoblotting with anti-GST antibody for Segment 3 and anti-6xHis antibody for BbPOTRA1-5. E. Whole cell lysate and co-purification from E. coli expressing OspC as well as BbPOTRA1-5, subjected to immunoblotting with anti-GST antibody for OspC (48 kDa) and anti-6xHis antibody for BbPOTRA1-5.