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. 2024 Aug 29;19(8):e0304839. doi: 10.1371/journal.pone.0304839

Fig 4. B. burgdorferi DUF490 interacts with the POTRA2 and POTRA3 domains of BamA.

Fig 4

All samples were subjected to immunoblotting with anti-GST antibody for GST fusions and anti-6xHis antibody for BbPOTRA fusions. Lower molecular weight bands (26 kDa) in anti-GST lanes correspond with the size of the GST tag. The black arrows next to blots indicate the GST fusion band and white arrows indicate the 6xHis fusion band. The colored arrows below the blots indicate proteins co-expressed in each assay. A. Whole cell lysate and co-purification from E. coli expressing BbPOTRA1 (P1, 9 kDa) and BbDUF490 (66kD), BbDUF490 segments (39 kDa), or OspC (48 kDa). BbPOTRA1 is highlighted in the AlphaFold v2.0 model of BbPOTRA1-5 on the right. B. Whole cell lysate and co-purification from E. coli expressing BbPOTRA2 (P2, 9 kDa) and DUF490, DUF490 segments, or OspC. BbPOTRA2 is highlighted in the AlphaFold v2.0 model of BbPOTRA1-5 on the right. C. Whole cell lysate and co-purification from E. coli expressing BbPOTRA3 (P3, 11 kDa) and BbDUF490, BbDUF490 segments, or OspC. BbPOTRA3 is highlighted in the AlphaFold v2.0 model of BbPOTRA1-5 on the right. D. Whole cell lysate and co-purification from E. coli expressing BbPOTRA4 (P4, 9 kDa) and DUF490, BbDUF490 segments, or OspC. BbPOTRA4 is highlighted in the AlphaFold v2.0 model of BbPOTRA1-5 on the right. E. Whole cell lysate and co-purification from E. coli expressing BbPOTRA5 (P5, 9 kDa) and BbDUF490, BbDUF490 segments, or OspC. BbPOTRA5 is highlighted in the AlphaFold v2.0 model of BbPOTRA1-5 on the right.