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. 2024 Aug 29;19(8):e0304839. doi: 10.1371/journal.pone.0304839

Fig 8. Salmonella DUF490 interacts with the POTRA domains of both TamA and BamA.

Fig 8

Lower molecular weight bands (26 kDa) in anti-GST lanes correspond with the size of the GST tag. The black arrows next to blots indicate the GST fusion band and white arrows indicate the 6xHis fusion band. A. Whole cell lysate and co-purification from E. coli expressing one of the following: StDUF490 (DUF, 66 kDa) or Pal (42 kDa) as well as StTamA POTRA1-3 (P1-3, 31 kDa), subjected to immunoblotting with anti-GST antibody for GST fusions and anti-6xHis antibody for StTamAPOTRA1-3. B. Whole cell lysate and co-purification from E. coli expressing one of the following: StDUF490 (DUF, 66 kDa) or Pal (42 kDa) as well as StBamA POTRA1-5 (P1-5, 45 kDa), subjected to immunoblotting with anti-GST antibody for GST fusions and anti-6xHis antibody for StBamAPOTRA1-5. C. Whole cell lysate and co-purification from E. coli expressing StDUF490 (DUF, 66 kDa) and one of the following: StBamA POTRA1-5 (P1-5, 45 kDa) or OspC (22 kDa), subjected to immunoblotting with anti-GST antibody for StDUF490 and anti-6xHis antibody for StBamA POTRA1-5 and OspC.