|
OspC ACYC BamHI F
|
GAGGGATCCTAATAATTCAGGGAAAGATGGGAATACATC
|
Forward primer for OspC into pACYCDuet-1. Nucleotide: 58-. |
|
B31 OspC EcoRI R
|
GAGGAATTCTTAAGGTTTTTTTGGACTTTCTGC
|
Reverse primer for OspC into pACYCDuet-1. Nucleotide: -630. |
|
DUF490 BamHI F
|
GAGGGATCCGGCGATTTTTCAATTGAAGGAAATGC
|
Forward primer for BbDUF490 and Segment 1 into pGEX-4T-1. Nucleotide: 3349-. |
|
DUF490-1 EcoRI R
|
GAGGAATTCTTACTTAATAATAAAATCATCTGTTTTTGTGTCAGA
|
Reverse primer for Segment 1 into pGEX-4T-1. Nucleotide: -3663. |
|
DUF490-2 BamHI F
|
GAGGGATCCGGAGATTTGAATATTGCAAG
|
Forward primer for Segment 2 into pGEX-4T-1. Nucleotide: 3664-. |
|
DUF490-2 EcoRI R
|
GAGGAATTCTTAACTTACTATTCCAATTGCCATTTCAGCA
|
Reverse primer for Segment 2 into pGEX-4T-1. Nucleotide: -4002. |
|
DUF490-3 BamHI F
|
GAGGGATCCGACATTGCTCTTGATTTTTTAATTCAACC
|
Forward primer for Segment 3 into pGEX-4T-1. Nucleotide: 4003-. |
|
DUF490 EcoRI R
|
GAGGAATTCTTAGTAATCAAACTCATAATTAACCAAAAAAAATGGAG
|
Reverse primer for BbDUF490 and Segment 3 into pGEX-4T-1. Nucleotide: -4329. |
|
P1 ACYC BamHI F
|
GAGGGATCCTAAGGGGAAAATAATAAAGGGTATTAATTTTG
|
Forward primer for BbPOTRA1 and BbPOTRA1-5 into pACYCDuet-1. Nucleotide: 82-. |
|
P1 EcoRI R
|
GAGGAATTCTTATTCTTTTACAATAAATGTAATAAAAAGATCCT
|
Reverse primer for BbPOTRA1 into pACYCDuet-1. Nucleotide: -303. |
|
P2 ACYC BamHI F
|
GAGGGATCCTAAATCTTTAGTTAATTCTGTTGTTTTTTCTG
|
Forward primer for BbPOTRA2 into pACYCDuet-1. Nucleotide: 304-. |
|
P2 EcoRI R
|
GAGGAATTCTTATCCAGCTACTATATTAAAAATAATATCAACTA
|
Reverse primer for BbPOTRA2 into pACYCDuet-1. Nucleotide: -540. |
|
P3 ACYC BamHI F
|
GAGGGATCCTCCCAAATATGTTGTTAAGGGGAT
|
Forward primer for BbPOTRA3 into pACYCDuet-1. Nucleotide: 541-. |
|
P3 EcoRI R
|
GAGGAATTCTTAGCCTTCTGAAAGAAAATATTTCAAAAAA
|
Reverse primer for BbPOTRA3 into pACYCDuet-1. Nucleotide: -822. |
|
P4 ACYC BamHI F
|
GAGGGATCCTAATGTTTTTAGATTTGGAAAGCTTG
|
Forward primer for BbPOTRA4 into pACYCDuet-1. Nucleotide: 823-. |
|
P4 EcoRI R
|
GAGGAATTCTTAATCCTTTTCTAAAATTTTAATTAACAAATCAAC
|
Reverse primer for BbPOTRA4 into pACYCDuet-1. Nucleotide: -1062. |
|
P5 ACYC BamHI F
|
GAGGGATCCTAAAGCTCATATTGAGTCTATTACTGTTT
|
Forward primer for BbPOTRA5 into pACYCDuet-1. Nucleotide: 1063- |
|
P5 EcoRI R
|
GAGGAATTCTTAATTACTTGTTGCTCGCTCCT
|
Reverse primer for BbPOTRA5 and BbPOTRA1-5 into pACYCDuet-1. Nucleotide: -1299. |
|
Pal BamHI F
|
GAGGGATCCAGCAATGACGGTAGCGAAGGCGGTATGCTGAAC
|
Forward primer for Pal into pGEX-4T-1. Nucleotide: 85-. |
|
Pal EcoRI R
|
GAGGAATTCTTAGTAAACCAGTACAGCGCGACG
|
Reverse primer for Pal into pGEX-4T-1. Nucleotide: -522. |
|
BamB ACYC BamHI F
|
GAGGGATCCTTCAAGCGAATCCATATTTTCACAAT
|
Forward primer for BbBamB into pACYCDuet-1. Nucleotide: 76-. |
|
BamB EcoRI R
|
GAGGAATTCTTATTCTTTAGTTAATTTTCTGTTTTCCAATTTCC
|
Reverse primer for BbBamB into pACYCDuet-1. Nucleotide: -1047. |
|
BamD ACYC BamHI F
|
GAGGGATCCTTATACGATTAACTTAGAAAAATTAACAAAAGAAAC
|
Forward primer for BbBamD into pACYCDuet-1. Nucleotide: 43-. |
|
BamD EcoRI R
|
GAGGAATTCTTATTTTTTATTATTTTCTATTTTATTTAATATTTTTTTAGCTAAAGG
|
Reverse primer for BbBamD into pACYCDuet-1. Nucleotide: -357. |
|
StDUF490 BamHI F
|
GAGGGATCCACACAACAAGGGCAAATTAACC
|
Forward primer for StDUF490 into pGEX-4T-1. Nucleotide: 2767-. |
|
StDUF490 EcoRI R
|
GAGGAATTCTTAAAACTCAAACTGATAGAGCAAATCAAGTG
|
Reverse primer for StDUF490 into pGGEX-4T-1. Nucleotide: -3777. |
|
StP1-3 ACYC BamHI F
|
GAGGGATCCAGCCGCAAATGTTCGTCTGAAAG
|
Forward primer for StTamA POTRA1-3 into pACYCDuet-1. Nucleotide: 61-. |
|
StP1-3 XhoI R
|
GCGCTCGAGTTATTCGGTTCGCGGCGAT
|
Reverse primer for StTamA POTRA1-3 into pACYCDuet-1. Nucleotide: -795. |
|
StP1-5 ACYC BamHI F
|
GAGGGATCCATTCGTAGTGAAGGACATTCATT
|
Forward primer for StBamA POTRA1-5 into pACYCDuet-1. Nucleotide: 70-. |
|
StP1-5 EcoRI R
|
GAGGAATTCTTAGCGCTCTTTCACCTTGTACACC
|
Reverse primer for StBamA POTRA1-5 into pACYCDuet-1. Nucleotide: -1063. |
