Fig 7.

(A) Pathogenicity of P. aeruginosa PA14 and the indicated P. aeruginosa clinical isolates (WT) or the cognate apaH deletion mutants (ΔapaH) in the lettuce leaf virulence assay. Bacterial viable cells (CFUs) per mg of lettuce midribs at three days post injection are shown. Five biological replicates for each strain were analyzed. A representative picture of the infected midribs is shown for each strain. (B) Reduction in infectivity, calculated as the ratio between the lethal dose 90% (LD₉₀) of the ΔapaH mutant and the LD₉₀ of the corresponding wild type strain, observed for apaH mutants of P. aeruginosa PA14 and the indicated P. aeruginosa clinical isolates in G. mellonella larvae. The dose-dependent survival curves used to calculate the LD₉₀ values and the corresponding R² values are shown in S10 Fig.