Extended Data Fig. 7: m⁶A regulates C9ORF72 repeat-containing RNA.

a, IGV profile showing the m⁶A peaks (generated by comparing MeRIP against input) in control and C9ORF72-ALS/FTD iPSNs. Red arrow head represents the repeat expansion. b, Western blotting of METTL14 in control or METTL14 knockdown i³Neurons. *non-specific band. c, The relative basal expression of NLuc RNA in the HeLa Flp-In cells expressing the reporters as described in Fig. 4b. *p=0.015 by two-tailed t test. d, The relative expression level of C9R-NLuc RNA upon knockdown of METTL14 in i³Neurons, measured by RT-qPCR and normalized to non-targeting shRNA control. ****p<0.0001 by two-tailed t test. (c,d) Points represent three biological replicates. Data are mean ± s.e.m.