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. Author manuscript; available in PMC: 2024 Aug 29.
Published in final edited form as: Nat Neurosci. 2023 Jun 26;26(8):1328–1338. doi: 10.1038/s41593-023-01374-9

Extended Data Fig. 10: m6A restoration reduces glutamate-induced excitotoxicity in C9ORF72-ALS/FTD iPSNs.

Extended Data Fig. 10:

a, RT-qPCR of METTL14 in C9ORF72-ALS/FTD iPSNs expressing exogenous METTL14 or GFP control. Data are mean ± s.e.m. Points represent individual cell lines. n=6 in each group. ***p=0.0006 by paired two-tailed t test. b, Western blotting of FTO in C9ORF72-ALS/FTD iPSNs upon knockdown of FTO by shRNA. *non-specific band. c,d, Representative images of Hoechst and propidium iodide (PI) staining of iPSNs in the glutamate-induced excitotoxicity assay. C9ORF72-ALS/FTD iPSNs were infected with lentivirus overexpressing METTL14 or GFP as negative control (c), or expressing FTO shRNA or non-targeting control shRNA (d).