Extended Data Fig. 1: m6A pathway-related gene expression in C9ORF72-ALS/FTD iPSNs.
a, Proteomic heatmap of the m6A pathway components in control (n=26) and C9ORF72-ALS/FTD (n=22) iPSNs. Data were normalized to the batch controls. b, Boxplots of the proteomic data in (a). Box plots indicate the interquartile range with the central line representing the median, and the vertical lines extend to the extreme values in the group. c, Western blotting (left) and quantification (right) of METTL3 and METTL14 expression in control and C9ORF72 iPSNs. Data are mean ± s.e.m. ***p=0.0008 and 0.0003, respectively, by two-tailed t test. d, Relative RNA expression of neuronal marker genes (MAP2, PSD95 and TUBB3) was measured by RT-qPCR in control and C9 iPSNs, showing comparable neuron maturity between control and C9. Points represent individual control or patient lines. n=6 in each group. Data are mean ± s.e.m. MAP2: p=0.304; PSD95: p=0.86; TUBB3: p=0.716, by two-tailed t test.