Figure 1:

Schematic of study to compare sparsentan and losartan in gddY mice. The 4-week-old gddY mice received sparsentan (900 or 1800 ppm) in their diet or losartan (10 or 30 mg/kg) in drinking water for 8 or 16 weeks, during which urine was collected every 2 weeks for albumin and creatinine determination. Blood pressure was recorded when mice were 8, 12 and 18 weeks old. After 8 weeks of treatment, a subgroup of mice were sacrificed, and the remainder were treated for a total of 16 weeks. Blood was taken for generation of plasma, and kidney samples were removed and processed for assessment of glomerulosclerosis, WT-1 positivity, glycocalyx (20 weeks age only) and gene expression. Created with Biorender.com by W.C. RT-PCR, real-time polymerase chain reaction.