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Antigen-specific CD4 T-cell function assays in long-term cryopreserved PBMC. (A) Representative flow plots of mitogen- and antigen-specific CD4 T cells, gated on day 2 cultured CD3+CD4+ T cells, detected by activation induced marker (AIM) assay (defined as CD25+CD134+ cells). (B) Representative flow plots of proliferating mitogen- and antigen-specific CD4 T cells, gated on day 7 cultured CD3+CD4+ T cells (defined as CD25 high Forward Scatter high blast cells).
