Extended Data Fig. 3. Regulation of colitis by NPM1 is independent of myeloid cells and T cells.
(a–h) Proportion of eosinophil, macrophage, neutrophil and dendritic cells (DCs) in lamina propria lymphocytes (LPLs) of Npm1+/+ and Npm1+/− mice under steady-state conditions (a–d) (Npm1+/+: n = 5 individual mice; Npm1+/−: n = 6 individual mice) and during DSS-induced colitis (e–h) (n = 6 individual mice) state are shown. (i–m) Colitis in Npm1+/+ and Npm1+/− mice was induced by DSS following administration with IgG or anti-CD11b blocking antibody. Deletion of CD11b+ cells by antibody (i). Representative images of colons (j), colon length (k) (n = 5 individual mice), colon histopathology on day 10 (l) and DAI (m) (n = 3 individual mice) are presented. Mice were injected with CD11b antibody (100 μg per mouse) every 2 days (from day −2 to day 6). Scale bars: 500 μm (up), 100 μm (down). (n–s) Proportion of TH17, Treg and γδT in LPLs of Npm1+/+ and Npm1+/− mice under steady-state conditions (n–p) (Npm1+/+: n = 5 individual mice; Npm1+/−: n = 6 individual mice) and during DSS-induced colitis (q–s) (n = 5 individual mice) state are shown. (t–x) DSS-induced colitis in Npm1+/+ and Npm1+/− mice was established following administration with IgG or anti-CD3 blocking antibody. Deletion of CD3+ cells by antibody (t). Representative images of colons (u), colon length (v) (n = 5 individual mice), colon histopathology (w) and DAI (x) (n = 3 individual mice) on day 10 are presented. Mice were injected with CD3 antibody (50 μg per mouse) once a day (from day −2 to day 6). Scale bars: 500 μm (up), 100 μm (down). Data in a–h, k, m, n–s, v and x are representative of two independent experiments, shown as the means ± s.e.m., and statistical significance was determined two-tailed unpaired Student’s t-test (**p < 0.01 and ****p < 0.0001).