Extended Data Fig. 6. Exacerbate enteritis in Rorc^cre/+Npm1^flox/flox mice under DSS is independent of T cells.

(a–n) Proportions of eosinophil, dendritic cells (DCs), macrophages, neutrophils, T_H17, T_reg and γδT in LPLs from Npm1^flox/flox and Rorc^cre/+Npm1^flox/flox mice under steady-state (a–d,i–k) and during DSS-induced colitis (e–h,l–n) (n = 5 individual mice). (o–q) Colitis in Npm1^flox/flox and Rorc^cre/+Npm1^flox/flox mice was induced by DSS following administration with IgG or anti-CD3 blocking antibody. Representative images of colons (o), colon length (p) and colon histopathology (q) on day 10 are presented (n = 5 individual mice). Mice were injected with CD3 antibody (50 μg per mouse) once a day (from day −2 to day 6). Scale bars: 500 μm (up), 100 μm (down). (r) Representative images of colons with tumors from Npm1^flox/flox and Rorc^cre/+Npm1^flox/flox on day 65 of the AOM/DSS CAC model. (s,t) Total number of tumors (s) and number of tumors larger than 2 mm (t) in Npm1^flox/flox and Rorc^cre/+Npm1^flox/flox mice (n = 4 individual mice). Data in a–n, p, s and t are representative of two independent experiments, shown as the means ± s.e.m., and statistical significance was determined two-tailed unpaired Student’s t-test (*p < 0.05, **p < 0.01 and ***p < 0.001).

Source data