Extended Data Fig. 7. NPM1 is essential for mitochondrial function in ILC3s.
(a–c) RT-PCR analysis of mRNA abundance of the indicated genes in macrophage (a), T cells (b) and epithelial (c), sorted from the LPLs of Npm1+/+ and Npm1+/− mice in steady state or at day 5 of administration of 2.5% DSS (n = 3 individual mice). (d–g) Cell mito stress test was performed with sorted colonic ILC3s from Npm1+/+ and Npm1+/− mice under steady-state. Representative oxygen consumption rate profile (OCR) (d), basal OCR (e), ATP production (f) and maximal respiration (g) are shown (n = 3 individual mice). (h–k) Cell mito stress test was conducted with sorted colonic ILC3s from wild-type (WT) mice on days 0, 5 and 10 of DSS-induced colitis. Representative OCR (h), basal OCR (i), ATP production (j) and maximal respiration (k) are presented (n = 3 individual mice). (l–t) Cell mito stress test was performed with sorted colonic macrophages (l–n), T cells (o–q), epithelial cells (r–t) from LPLs of Npm1+/+ and Npm1+/− mice at day 5 of administration of 2.5% DSS (n = 4 individual mice). (u,v) Mitochondrial membrane potential was assessed with the indicator JC-1 (u) and TMRE (v) in isolated colonic ILC3s from Npm1+/+ and Npm1+/− mice in steady state (n = 5 individual mice). (w–y) Npm1flox/flox and Rorccre/+Npm1flox/flox mice were treated with bezafibrate (i.g., 10 mg/kg) and administered 2.5% DSS for 7 days followed by 3 days of recovery. Representative colon images (w), colon length (x) and colon histopathology (y) are shown (n = 5 individual mice). Scale bars: 500 μm (up), 100 μm (down). Data are representative of two independent experiments, shown as the means ± s.e.m., and statistical significance was determined by two-way ANOVA (a–c) and two-tailed unpaired Student’s t-test (e–g,i–v,x) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).