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. 2024 Aug 7;25(9):1593–1606. doi: 10.1038/s41590-024-01915-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Thymic cellularity of female C57BL/6 mice at 2, 6, 9, 12 or 19+ months of age. b-c, Representative images of hematoxylin and eosin (H&E) stained mouse thymi from 2, 6, 8-9, 13, and 19+mo female C57BL/6 mice (b) used to calculate ratio of cortical (dark) to medullary (light) region (c). In (c), each dot represents a biological replicate. d, Flow cytometric analysis of enzymatically digested thymus and absolute cell numbers for major cell types (TECs; cTECs and mTECs; ECs and FBs) in 2, 6, 9, 12, and 18+ mo female C57BL/6 mice. Summary data represents mean ± SEM; each dot represents an individual biological replicate; statistics were generated for a, c, and d using one-way ANOVA with the Dunnett correction for multiple comparisons.