Table 4.
Primers for detection of GyV.
| Virus | GenBank (size) | Primer sequence (5′→3′) | Position (size) | Source |
|---|---|---|---|---|
| CAV |
NC_001427 (2,319 bp) |
F: GACTGTAAGATGGCAAGACGAGCTC R: GGCTGAAGGATCCCTCATTC |
823–1,498 (676 bp) |
References |
| GyG1 |
HM590588 (2,383 bp) |
F:CGTGTCCGCCAGCAGAAAC R:GGTAGAAGCCAAAGCGTCCAC |
656–1,001 (346 bp) |
References |
| GyH1 |
NC_017091 (2,359 bp) |
F:GACACAGACTGCGACGAAGA R:ATGCTCCTGGCTGTCTAGAT |
968–1,399 (432 bp) |
References |
| GyH3 |
JX310702 (2034 bp) |
F: GTGGTATCGAAGTGGAAAGTACC R: CCCCCTGATACATACTGTACATA |
1,061–1,345 (285 bp) |
References |
| GyH2 |
KF294862 (2,282 bp) |
F: TCCATCATTCTCAGCGTTAT R: CGTAGATTGTGGCTATTGTG |
1,875–2,092 (218 bp) |
Design |
| GyH4 |
KF294861 (2020 bp) |
F:CAAGATTCGTATTGGCAAGT R:CATTCACTCCGCACCTTA |
1,580–1,953 (374 bp) |
Design |
| GyG2 |
KM111536 (2,439 bp) |
F:CGAAGTGTAAGCCGAAGG R:CGTTGTTCCACCAGTTGA |
2,035–2,374 (340 bp) |
Design |
| GyF1 |
KR137527 (2,218 bp) |
F:TTGACTGGTGGAGATGGT R:CCTGTGATTGTGCTGGTT |
1,276–1,606 (331 bp) |
Design |
| GyH5 |
MT318123 (2,215 bp) |
F:CTGCCTTATACTGGTGGAAT R:TTGGTAGTTCTTGCTGGTAA |
1,281–1,761 (481 bp) |
Design |
| GyA1 |
MH016740 (2,195 bp) |
F:CCTCATTCGGAACACTCTC R:TGGTTGGTCTTGGTCTGT |
1,745–2,016 (272 bp) |
Design |
| GyHy1 |
MH378452 (2,394 bp) |
F:CCATCCTTCGCAACACTAT R:CACCTTGACTGTATCTTAACTG |
1,569–1,900 (332 bp) |
Design |
| GyM1 |
MH638372 (1873 bp) |
F:ACCACCGAAGACGATACT R:TAGGAGGTTGCCGAATGA |
750–1,006 (257 bp) |
Design |
| GyPi1 |
MN816164 (2,573 bp) |
F:TAAGAGCAGCGAGCATTG R:TGGTAGCGGATACATTAGTG |
1,414–1,691 (278 bp) |
Design |
| GyA2 |
MT318125 (2,195 bp) |
F: AGACATAGACGACGATTCC R: AGTTCATTACTGCTCTCCAT |
615–912 (298 bp) |
Design |
| GyPh1 |
OK665854 (2,353 bp) |
F:ATACGAATGGTGGAGATGG R:TAGTGTGGCGAATGATGG |
1,431–1,908 (478 bp) |
Design |
| GyMe1 |
OQ064396 (2,573 bp) |
F:TAAGAGCAGCGAGCATTG R:TGGTAGCGGATACATTAGTG |
1,414–1,691 (278 bp) |
Design |
Designed primers for GyV detection using primer 6.0 software.