Fig. 2. Adipose-Phd2 deletion in females sustains higher energy expenditure at thermoneutrality.
A P2KOad (KO, purple) mice housed at 20–21 °C (RT, n = 9) when acutely switched to 28–29 °C (TN, 7 days) sustain similar body weight (BW) to control (C, black, n = 7) littermates but B have higher energy expenditure (n = 6/group). C Similar respiratory exchange ratio (n = 6/group) and D activity levels (n = 6/group). E Control mice reduce, but KO sustain food intake at TN (n = 6/group). F Similar fat and lean mass measured by TD-NMR (C: n = 7 and KO: n = 9) in both genotypes. G Similar BAT mass (*** indicates comparisons of RT with TN; RT C: n = 8, KO: n = 6 and TN: n = 6/group) and H WAT mass (i inguinal, p parametrial, m mesenteric, RT: n = 3/group; TN C: n = 7 and KO: n = 9) in both genotypes. n indicates biological replicates. Data are presented as mean + /− SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by paired (A–F) or unpaired (G, H) Student t test (two-tailed). For EE, ANCOVA was performed with BW as covariance. Source data are provided as a Source Data file.