Fig. 3. Metabolic parameters in male and female P2KO^ad mice housed at room temperature (RT) and thermoneutrality (TN).

A, B Plasma non-esterified fatty acids, C, D glycerol, E, F glucose and G, H insulin levels. Males, NEFA: control RT n = 6, TN n = 10, KO RT n = 7 TN n = 10, glycerol: n = 7/group, glucose: control RT n = 6 TN n = 13, KO RT n = 7, TN n = 12, insulin: RT n = 5/group, TN n = 7/group). Females, NEFA: control n = 5, KO n = 6; glycerol: control n = 5, KO n = 5; glucose: control n = 7, KO n = 8; insulin: control n = 6, KO n = 8). Data are presented as mean + /− SEM. N represents biological replicates. *P < 0.05, **P < 0.01 by Student t test (two-tailed). Source data are provided as a Source Data file.