Fig. 6. P2KO^ad mice retain higher EE after high fat feeding at thermoneutrality.

A P2KO^ad (orange, n = 5) and control (C, light blue, n = 5) mice were fed high fat diet (58% kcal from fat) while housed at TN (28–29 °C) for 8 weeks had similar body weight gain. B Higher energy expenditure in high fat fed P2KO^ad at TN. C Similar respiratory exchange ratio, D activity levels, E fat mass gain by TD-NMR, F WAT and G BAT weights after high fat feeding. H Plasma glucose, I NEFA and J insulin levels. K mRNA levels of HIF-target and thermogenic genes in BAT after high fat feeding at TN. Data are presented as mean + /− SEM. *P < 0.05, **P < 0.01, Student t test (two-tailed). ns; no statistically significant. Source data are provided as a Source Data file.