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. 2024 Aug 29;15:7514. doi: 10.1038/s41467-024-51804-w

Fig. 4. Expression of L-ENA genes is concomitant with sporulation.

Fig. 4

a Chromosomal organization of exsL, l-bclA, and ena3A, and primers used in standard PCR using cDNA template; b The L-ENA genes l-bclA and ena3A form an operon. Agarose gel electrophoresis (1%) of PCR products conducted on cDNA from NVH 0075-95 culture (4, 8, 12, and 16 h). Primer combinations and expected product sizes are shown in (a). Genomic DNA (gDNA) from NVH 0075-95 and cDNA prepared from its isogenic Δena1ABC/Δena3A mutant were used as positive and negative controls for the PCR, respectively; c Expression of L-ENA genes relative to rpoB during vegetative growth and sporulation. Data are presented as mean values with error bars representing the standard deviation of three independent experiments, each with three technical replicates. Source data are provided as a Source Data file.