Fig. 5. Substrate and adaptor densities associated with Cdc48.

A Rigid-body fitting of PP1 (Glc7-Sds22, PDB 6OBN) and UBX (PDB 6OPC) crystal structures into Cdc48 reconstruction containing PP1 density. Unassigned density in purple above the central pore of the hexamer likely represents a mixture of adaptor and substrate density. B Model and density of Shp1 D1B (Cdc48 ATPase Domain 1 binding beta-strand) motif associated with D1 large subdomains of subunits B, C, and D. Putative unstructured substrate/adaptor density colored in purple, as in A. C, D Close-up model and density of Shp1 D1B motif associated with subunit C.